Exploring hydrogels based on the self-assembly of a Fmoc-based tripeptide: physicochemical characterization and antibacterial properties
Exploring hydrogels based on the self-assembly of a Fmoc-based tripeptide: physicochemical characterization and antibacterial properties
Informations :
Type : Soutenance de thèse
Date : 2024-04-05
Heure : 09:30
Lieu : Amphithéâtre Henri Benoît
Titre : Exploring hydrogels based on the self-assembly of a Fmoc-based tripeptide: physicochemical characterization and antibacterial properties
Conférencier : LOTH Capucine
Appartenance : Institut Charles Sadron
Invité par : Boulmedais Fouzia
Description :
Hydrogels are 3D networks of fibers that retain large amounts of water when swollen. Due
to their biocompatibility, they are increasingly used for drug delivery. To develop
antibacterial peptide-based hydrogels, this dissertation presents two studies based on the
use of a fluorenylmethoxycarbonyl (Fmoc)-protected phosphorylated tripeptide that can
self-assemble into a hydrogel. In the first study, different preparation conditions (pH, salt,
presence of polysaccharide) were investigated to obtain a self-healing and antibacterial
hydrogel capable of releasing an antibiotic, florfenicol. In the second study, a solid-phase
peptide and phosphoramidite synthesis strategies were combined to add florfenicol to the
Fmoc-protected tyrosine phosphate via a phosphodiester, which can be cleaved by nucleases
produced by bacteria. Encouraging results showed the formation of the targeted compound,
paving the way for the design of a self-defensive antibacterial peptide.
Keywords: Fmoc-FF, peptide-based hydrogels, electrostatic assemblies, antibacterial
hydrogels, drug delivery matrices